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Dinitroaniline herbicide resistance in a multiple‐resistant Lolium rigidum population
Author(s) -
Chen Jinyi,
Yu Qin,
Owen Mechelle,
Han Heping,
Powles Stephen
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4790
Subject(s) - lolium rigidum , trifluralin , population , pendimethalin , biology , weed , acetolactate synthase , oryzalin , cross resistance , agronomy , herbicide resistance , weed control , genetics , gene , medicine , microtubule , environmental health
BACKGROUND The pre‐emergence dinitroaniline herbicides (such as trifluralin and pendimethalin) are vital to Australian no‐till farming systems. A Lolium rigidum population collected from the Western Australian grain belt with a 12‐year trifluralin use history was characterised for resistance to dinitroaniline, acetyl CoA carboxylase (ACCase)‐ and acetolactate synthase (ALS)‐inhibiting herbicides. Target‐site resistance mechanisms were investigated. RESULTS This L. rigidum population exhibited 32‐fold resistance to trifluralin, as compared with the susceptible population. It also displayed 12‐ to 30‐fold cross‐resistance to other dinitroaniline herbicides (pendimethalin, ethalfluralin and oryzalin). In addition, this population showed multiple resistance to commonly used post‐emergence ACCase‐ and ALS‐inhibiting herbicides. Two target‐site α‐tubulin gene mutations (Val‐202‐Phe and Thr‐239‐Ile) previously documented in other dinitroaniline‐resistant weed species were identified, and some known target‐site mutations in ACCase (Ile‐1781‐Leu, Asp‐2078‐Gly and Cys‐2088‐Arg) and ALS (Pro‐197‐Gln/Ser) were found in the same population. An agar‐based Petri dish screening method was established for the rapid diagnosis of resistance to dinitroaniline herbicides. CONCLUSION Evolution of target‐site resistance to both pre‐ and post‐emergence herbicides was confirmed in a single L. rigidum population. The α‐tubulin mutations Val‐202‐Phe and Thr‐239‐Ile, documented here for the first time in L. rigidum , are likely to be responsible for dinitroaniline resistance in this population. Early detection of dinitroaniline herbicide resistance and integrated weed management strategies are needed to maintain the effectiveness of dinitroaniline herbicides. © 2017 Society of Chemical Industry