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Changes in field dose–response curves for demethylation inhibitor (DMI) and quinone outside inhibitor (QoI) fungicides against Zymoseptoria tritici , related to laboratory sensitivity phenotyping and genotyping assays
Author(s) -
Blake Jonathan J,
Gosling Paul,
Fraaije Bart A,
Burnett Fiona J,
Knight Stuart M,
Kildea Steven,
Paveley Neil D
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4725
Subject(s) - fungicide , biology , demethylation , genotyping , microbiology and biotechnology , botany , genetics , genotype , gene , gene expression , dna methylation
BACKGROUND Insensitivity of Zymoseptoria tritici to demethylation inhibitor (DMI) and quinone outside inhibitor (QoI) fungicides has been widely reported from laboratory studies, but the relationships between laboratory sensitivity phenotype or target site genotype and field efficacy remain uncertain. This article reports field experiments quantifying dose–response curves, and investigates the relationships between field performance and in vitro half maximal effective concentration (EC 50 ) values for DMIs, and the frequency of the G143A substitution conferring QoI resistance. RESULTS Data were analysed from 83 field experiments over 21 years. Response curves were fitted, expressed as percentage control, rising towards an asymptote with increasing dose. Decline in DMI efficacy over years was associated with a decrease in the asymptote, and reduced curvature. Field ED 50 values were positively related to in vitro EC 50 values for isolates of Z. tritici collected over a 14‐year period. Loss of QoI efficacy was expressed through a change in asymptote. Increasing frequency of G143A was associated with changes in field dose–response asymptotes. CONCLUSION New resistant strains are often detected by resistance monitoring and laboratory phenotyped/genotyped before changes in field performance are detected. The relationships demonstrated here between laboratory tests and field performance could aid translation between laboratory and field for other fungicide groups. © 2017 Society of Chemical Industry