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Bioassay‐guided isolation of potent aphicidal Erythrina alkaloids against Aphis gossypii from the seed of Erythrina crista‐galli L
Author(s) -
Wang Delong,
Xie Na,
Yi Shandong,
Liu Chuanyuan,
Jiang Hui,
Ma Zhiqing,
Feng Juntao,
Yan He,
Zhang Xing
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4698
Subject(s) - erythrina , aphis gossypii , bioassay , aphid , biology , botany , traditional medicine , homoptera , pest analysis , aphididae , medicine , genetics
BACKGROUND The cotton aphid ( Aphis gossypii Glover) is one of the most invasive pests of cotton. Many botanical phytochemicals have a long history as a source of insecticides, and as templates for new insecticides. This study was undertaken to isolate aphicidal compounds from the seeds of Erythrina crista‐galli L. using the bioassay‐guided isolation method. RESULTS Three novel and 11 known Erythrina alkaloids were isolated. Erysodine ( 9 ), erysovine ( 10 ), erysotrine ( 8 ) and erythraline ( 11 ) showed moderate to excellent aphicidal activity with LD 50 values of 7.48, 6.68, 5.13 and 4.67 ng aphid –1 , respectively. The Potter spray tower bioassay gave corresponding LC 50 values of 186.81, 165.35, 163.74 and 112.78 µg ml –1 . A unique substructure, which presents an sp 3 methylene at C‐8, a non‐oxygenated site at N‐9 and a conjugated dienes group (Δ 1,2 and Δ 6,7 ), plays a crucial role in the aphicidal activity. Application of erythraline ( 11 ) led to different increases in the activities of superoxide dismutase, catalase and glutathione S ‐transferase. CONCLUSION The study demonstrated that the Erythrina alkaloids erysodine ( 9 ), erysovine ( 10 ), erysotrine ( 8 ) and erythraline ( 11 ) have potential use as botanical aphicides for commercial application, or as templates for the development of new insecticides. © 2017 Society of Chemical Industry