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Reversing resistance to tembotrione in an Amaranthus tuberculatus (var. rudis ) population from Nebraska, USA with cytochrome P450 inhibitors
Author(s) -
Oliveira Maxwel C,
Gaines Todd A,
Dayan Franck E,
Patterson Eric L,
Jhala Amit J,
Knezevic Stevan Z
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4697
Subject(s) - mesotrione , biology , malathion , piperonyl butoxide , cytochrome p450 , population , botany , biochemistry , enzyme , agronomy , pesticide , atrazine , demography , sociology
BACKGROUND A population of Amaranthus tuberculatus (var. rudis ) was confirmed resistant to 4‐hydroxyphenylpyruvate dioxygenase (HPPD)‐inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non‐target‐site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD‐inhibitor herbicides on the HPPD‐inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD‐R). RESULTS Enhanced metabolism via cytochrome P450 enzymes is the mechanism of resistance in HPPD‐R. Amitrole partially restored the activity of mesotrione, whereas malathion, amitrole, and piperonyl butoxide restored the activity of tembotrione and topramezone in HPPD‐R. Although corn was injured through malathion followed by mesotrione application a week after treatment, the injury was transient, and the crop recovered. CONCLUSION The use of cytochrome P450 inhibitors with tembotrione may provide a new way of controlling HPPD‐inhibitor resistant A. tuberculatus , but further research is needed to identify the cytochrome P450 candidate gene(s) conferring metabolism‐based resistance. The results presented here aid to gain an insight into non‐target‐site resistance weed management strategies. © 2017 Society of Chemical Industry