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Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis
Author(s) -
Zhou Dan,
Duan Baiyun,
Sun Yan,
Ma Lei,
Zhu Changliang,
Shen Bo
Publication year - 2017
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4649
Subject(s) - biology , genome , gene , deltamethrin , sequence alignment , peptide sequence , transcriptome , biochemistry , amino acid , genetics , computational biology , gene expression , pesticide , ecology
BACKGROUND The insect cuticle protects against environmental stresses such as insecticides, physical injury, dehydration and pathogenic microorganisms. Structural cuticular proteins (CPs) are the primary components of the cuticle, and are of interest for physiology‐based pest management methods. Anopheles sinensis CPs are poorly characterised at present, and therefore we performed whole‐genome sequence analysis and re‐analysed available transcriptome data to identify potential insecticide resistance‐associated CPs. RESULTS Genome analysis revealed 238 putative CPs belonging to 11 different families; 136 proteins possessing a Rebers and Riddiford motif (CPR), 12 CPs analogous to peritrophins with a single chitin‐binding domain (CPAP1), eight CPs analogous to peritrophins with three chitin‐binding domains (CPAP3), four proteins with a 44 amino acid motif (CPF), five CPF‐like (CPFL) proteins, nine Tweedle proteins, three proteins of low complexity with alanine residues (CPLCA), 26 proteins of low complexity with conserved glycine residues (CPLCG), seven proteins of low complexity with invariant W residues (CPLCW), 27 proteins of low complexity with proline residues (CPLCP), and one protein with two or three copies of a C‐X ‐C motif (CPCFC). Two CPs belonging to the CPR and CPAP3 families were over‐expressed in a deltamethrin‐resistant strain. CONCLUSION These results establish an information framework for An. sinensis CPs that provides a basis for further molecular research. © 2017 Society of Chemical Industry