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A quantitative assay for Amaranthus palmeri identification
Author(s) -
Murphy Brent P,
Plewa Diane E,
Phillippi Elizabeth,
Bissonnette Suzanne M,
Tranel Patrick J
Publication year - 2017
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4632
Subject(s) - biology , internal transcribed spacer , polymerase chain reaction , botany , ribosomal rna , genetics , gene
BACKGROUND Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS Markers were developed for A. palmeri ‐specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single‐plant samples, simulated mixed‐plant samples and seed mixtures. CONCLUSION A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds. © 2017 Society of Chemical Industry