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The effects of phytochemical pesticides on the growth of cultured invertebrate and vertebrate cells
Author(s) -
Salehzadeh Areaf,
Jabbar Abdul,
Jennens Lynn,
Ley Steven V,
Annadurai Ramasamy S,
Adams Roger,
Strang Robin H C
Publication year - 2002
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.449
Subject(s) - azadirachtin , rotenone , biology , phytochemical , terpenoid , pesticide , cell growth , cell culture , insect growth regulator , invertebrate , growth inhibition , in vitro , toxicity , insect , toxicology , botany , biochemistry , chemistry , ecology , mitochondrion , genetics , organic chemistry
A range of cultured cells of invertebrate and vertebrate origin was grown in the presence of a number of phytochemical pesticides to test the effect of the latter on cell proliferation. The main observation was that azadirachtin was a potent inhibitor of insect cell replication, with an EC 50 of 1.5 × 10 −10 M against Spodoptera cells and of 6.3 × 10 −9 M against Aedes albopictus cells, whilst affecting mammalian cells only at high concentrations (>10 −4 M ). As expected, the other phytochemical pesticides, except for rotenone, had little effect on the growth of the cultured cells. Rotenone was highly effective in inhibiting the growth of insect cells (EC 50 :10 −8 M ) but slightly less toxic towards mammalian cells (EC 50 :2 × 10 −7 M ). Neem terpenoids other than azadirachtin and those very similar in structure significantly inhibited growth of the cell cultures, but to a lesser degree. The major neem seed terpenoids, nimbin and salannin, for example, inhibited insect cell growth by 23% and 15%, respectively. © 2002 Society of Chemical Industry