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A glycoprotein α ‐amylase inhibitor from Withania somnifera differentially inhibits various α ‐amylases and affects the growth and development of Tribolium castaneum
Author(s) -
Kasar Sainath S,
Marathe Kiran R,
Bhide Amey J,
Herwade Abhijeet P,
Giri Ashok P,
Maheshwari Vijay L,
Pawar Pankaj K
Publication year - 2017
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4467
Subject(s) - withania somnifera , amylase , biology , pest analysis , helicoverpa armigera , red flour beetle , food science , biochemistry , botany , enzyme , insect , larva , medicine , alternative medicine , pathology
Abstract BACKGROUND Identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. In particular, plant‐derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option. RESULTS A glycoprotein having non‐competitive α ‐amylase inhibitory activity with a molecular weight of 8.3 kDa was isolated and purified from seeds of Withania somnifera α ‐amylase inhibitor ( WSAI ). Its mass spectrometry analysis revealed 59% sequence coverage with Wrightide II ‐type α‐amylase inhibitor from Wrightia religiosa . A dose‐dependent inhibition of α ‐amylases from Aspergillus oryzae , Bacillus subtilis , Helicoverpa armigera and Tribolium castaneum was recorded. Interestingly, WSAI did not inhibit human salivary α ‐amylase significantly. When adults of T. castaneum were fed with WSAI (1.6 mg g −1 ), decrease in consumption, growth and efficiency of conversion of ingested food was evident, along with over fourfold increases in feeding deterrence index. A decline in larval residual α ‐amylase activity after feeding of WSAI resulted in a reduction in longevity of T. castaneum . CONCLUSION The study reflects the significance of WSAI in affecting the overall growth and development of T. castaneum . Pre‐ and post‐harvest pest resistive capability makes WSAI a potential candidate for insect pest management. Further, the effectiveness of this inhibitor could be explored either in formulations or through a transgenic approach. © 2016 Society of Chemical Industry