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Study of the efficiency of anticoagulant rodenticides to control Mus musculus domesticus introgressed with Mus spretus Vkorc1
Author(s) -
Goulois Joffrey,
Hascoët Claire,
Dorani Khedidja,
Besse Stéphane,
Legros Lionel,
Benoit Etienne,
Lattard Virginie
Publication year - 2017
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4319
Subject(s) - biology , introgression , genetics , phenotype , vitamin k epoxide reductase , house mice , gene , genotype , context (archaeology) , vkorc1 , zoology , cyp2c9 , paleontology
BACKGROUND Antivitamin K anticoagulant ( AVK ) rodenticides are commonly used to control rodent pests worldwide. They specifically inhibit the VKORC1 enzyme essential for the recycling of vitamin K, and thus prevent blood clotting and cause death by haemorrhage. Numerous mutations or polymorphisms of the Vkorc1 gene were reported in rodents, and some led to resistance to rodenticides. In house mice ( Mus musculus domesticus ), adaptive introgression of the Vkorc1 gene from the Algerian mouse ( Mus spretus ) was reported. This adaptive introgression causes the substitution of four amino acids in M. musculus domesticus . RESULTS The consequences of introgression were assessed by (i) the characterisation of the in vivo resistant phenotype of adaptive Vkorc1 spr ‐introgressed mice, (ii) the characterisation of the ex vivo resistance phenotype of the liver VKOR activity and (iii) the comparison of these results with the properties of recombinant VKORC1 spr protein expressed in yeast. The resistance factor (from 1 to 120) induced by the four introgressed polymorphisms obtained using these three approaches was dependent on the AVKs used but were highly correlated among the three approaches. CONCLUSION The four introgressed polymorphisms were clearly the cause of the strong resistant phenotype observed in the field. In the context of strong selection pressure due to the extensive use of AVKs , this resistant phenotype may explain the widespread distribution of this genotype from Spain to Germany. © 2016 Society of Chemical Industry