Premium
Phenotypical and biochemical characterisation of resistance for parasitic weed ( Orobanche foetida Poir.) in radiation‐mutagenised mutants of chickpea
Author(s) -
Brahmi Ines,
Mabrouk Yassine,
Brun Guillaume,
Delavault Philippe,
Belhadj Omrane,
Simier Philippe
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4278
Subject(s) - biology , phenylalanine ammonia lyase , mutant , botany , germination , weed , striga , horticulture , peroxidase , gene , enzyme , biochemistry
BACKGROUND Some radiation‐mutagenised chickpea mutants potentially resistant to the broomrape, Orobanche foetida Poir., were selected through field trials. The objectives of this work were to confirm resistance under artificial infestation, in pots and mini‐rhizotron systems, and to determine the developmental stages of broomrape affected by resistance and the relevant resistance mechanisms induced by radiation mutagenesis. RESULTS Among 30 mutants tested for resistance to O. foetida , five shared strong resistance in both pot experiments and mini‐rhizotron systems. Resistance was not complete, but the few individuals that escaped resistance displayed high disorders of shoot development. Results demonstrated a 2–3‐fold decrease in stimulatory activity of root exudates towards broomrape seed germination in resistant mutants in comparison with non‐irradiated control plants and susceptible mutants. Resistance was associated with an induction of broomrape necrosis early during infection. When infested, most of the resistant mutants shared enhanced levels of soluble phenolic contents, phenylalanine ammonia lyase activity, guaiacol peroxidase activity and polyphenol oxidase activity, in addition to glutathione and notably ascorbate peroxidase gene expression in roots. CONCLUSION Results confirmed enhanced resistance in chickpea radiation‐mutagenised mutants, and demonstrated that resistance is based on alteration of root exudation, presumed cell‐wall reinforcement and change in root oxidative status in response to infection. © 2016 Society of Chemical Industry