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Movement and survival of Busseola fusca (Lepidoptera: Noctuidae) larvae within maize plantings with different ratios of non‐ Bt and Bt seed
Author(s) -
Erasmus Annemie,
Marais Jaco,
Van den Berg Johnnie
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4273
Subject(s) - noctuidae , lepidoptera genitalia , biology , agronomy , bt cotton , genetically modified maize , larva , botany , genetically modified crops , genetics , transgene , gene
BACKGROUND Products of plant biotechnology, for example genetically modified Bt maize, provide useful tools for pest management. The benefits provided by insect‐resistant plants are, however, threatened by the evolution of resistance by target pest species. The high‐dose/refuge insect resistance management strategy ( IRM ) as well as seed mixtures are globally used as IRM strategies. Busseola fusca (Lepidoptera: Noctuidae), the target stem borer of Bt maize in Africa, evolved resistance to Bt maize expressing Cry1Ab protein in South Africa. Owing to high larval mobility and subsequent sublethal exposure of larvae moving between non‐ Bt and Bt plants, more rapid resistance evolution has been proposed as a possibility with deployment of seed mixture strategies. RESULTS Laboratory and field studies were conducted to study B . fusca larval mobility. In the laboratory, different scenarios of B. fusca larval movement between single‐gene ( Cry1Ab ) and stacked‐trait ( Cry1A .105 and Cry2Ab2 ) Bt maize were studied. Data on larval survival and mass over time indicated that Cry proteins do not kill larvae above certain developmental stages. A 2 year field study with the single gene and the stacked event was conducted using seed mixtures containing 5, 10, 15 and 20% non‐ Bt seed as well as a control treatment (non‐ Bt seed only). CONCLUSION Larval movement continued for 5 weeks and resulted in a significant incidence of Bt and non‐ Bt damaged plants, indicating that the movement behaviour of B. fusca is of such a nature that seed mixtures as an IRM strategy may not be effective to delay resistance evolution. © 2016 Society of Chemical Industry