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F 1 ‐ATP synthase α ‐subunit: a potential target for RNAi ‐mediated pest management of Locusta migratoria manilensis
Author(s) -
Hu Jun,
Xia Yuxian
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4185
Subject(s) - atp synthase , rna interference , pest analysis , atpase , biology , oligomycin , microbiology and biotechnology , v atpase , transcription (linguistics) , protein subunit , biochemistry , gene , enzyme , rna , botany , linguistics , philosophy
BACKGROUND The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase ( F 0 F 1 ‐ATPase ) uses proton or sodium motive force to produce 90% of the cellular ATP , and the α ‐subunit of F 1 ‐ATP synthase ( ATP5A ) is vital for F 1 ‐ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi ‐mediated pest management of L. migratoria . RESULTS Lm‐ ATP5A was cloned and characterised. Lm‐ ATP5A is expressed in all tissues. Injection of 100 ng of the double‐stranded RNA of ATP5A ( dsATP5A ) knocked down the transcription of the target gene and caused mortality in 1.5–5 days. The Lm‐ ATP5A protein level, the oligomycin‐sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection. CONCLUSION These findings demonstrated the essential roles of Lm‐ ATP5A in L. migratoria and identified it as a potential target for insect pest control. © 2015 Society of Chemical Industry