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Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose
Author(s) -
Kim Jeong Do,
Jeon Byeong Jun,
Han Jae Woo,
Park Min Young,
Kang Sin Ae,
Kim Beom Seok
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4181
Subject(s) - bacillus amyloliquefaciens , pepper , biology , cucumis , strain (injury) , biological pest control , horticulture , botany , bacteria , genetics , anatomy
BACKGROUND Endophytic bacteria are viewed as a potential new source of biofungicides because they have beneficial characteristics as control agents for plant disease. This study was performed to examine the endophytic feature and disease control efficacy of Bacillus amyloliquefaciens strain GYL4 and to identify the antifungal compounds produced by this strain. RESULTS B. amyloliquefaciens strain GYL4 was isolated from leaf tissue of pepper plants ( Capsicum annuum L.). Anthracnose symptoms were markedly reduced in the leaves of pepper plants colonised by GYL4 . An egfp ‐expressing strain of GYL4 ( GYL4 ‐ egfp ) was constructed and reintroduced into pepper plants, which confirmed its ability to colonise the internal tissues of pepper plants. GYL4 ‐ egfp was observed in the root and stem tissues 4 days after treatment and abundantly found in the internal leaf tissue 9 days after treatment. Bacillomycin derivatives purified from the culture extract of GYL4 displayed control efficacy on anthracnose development in cucumber ( Cucumis sativus L. cv. Chunsim). CONCLUSION The present study is the first report on evaluation of the endophytic and systemic nature of B. amyloliquefaciens strain GYL4 and its potential as a biocontrol agent for anthracnose management. © 2015 Society of Chemical Industry