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Detection of the F129L mutation in the cytochrome b gene in Phakopsora pachyrhizi
Author(s) -
Klosowski Ana C,
May De Mio Louise L,
Miessner Simone,
Rodrigues Ronaldo,
Stammler Gerd
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4099
Subject(s) - phakopsora pachyrhizi , genetics , mutation , biology , pyrosequencing , gene , soybean rust , cytochrome b , silent mutation , gene mutation , microbiology and biotechnology , fungicide , mitochondrial dna , botany , missense mutation
BACKGROUND Asian soybean rust, caused by Phakopsora pachyrhizi , is mostly controlled by demethylation inhibitor ( DMI ) and quinone outside inhibitor ( QoI ) fungicides. Mutations in the cytochrome b ( CYTB ) gene can lead to pathogen resistance to QoIs . The occurrence of the mutations in codons 129, 137 and 143 in the CYTB gene was investigated, and a pyrosequencing assay was developed for rapid and quantitative detection of the F129L mutation. RESULTS Molecular analysis of the CYTB gene showed the presence of the F129L mutation in field samples and monouredinial isolates, while other mutations ( G143A and G137R ) were not found. The pyrosequencing was an effective method for quantitative detection of the F129L mutation, and many of the P. pachyrhizi samples showed high frequency of F129L . CONCLUSION This is the first report of the occurrence of the F129L mutation in P. pachyrhizi . The practical relevance of this mutation for field efficacy of QoIs needs further investigation. © 2015 Society of Chemical Industry