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Genetic basis of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to the chitin synthesis inhibitor lufenuron
Author(s) -
Nascimento Antonio Rogério Bezerra do,
Farias Juliano Ricardo,
Bernardi Daniel,
Horikoshi Renato Jun,
Omoto Celso
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4057
Subject(s) - lufenuron , biology , spodoptera , lepidoptera genitalia , genetics , noctuidae , diflubenzuron , botany , gene , larva , recombinant dna
BACKGROUND An understanding of the genetic basis of insect resistance to insecticides is important for the establishment of insect resistance management ( IRM ) strategies. In this study we evaluated the inheritance pattern of resistance to the chitin synthesis inhibitor lufenuron in Spodoptera frugiperda . RESULTS The LC 50 values (95% CI ) were 0.23 µg lufenuron mL −1 water (ppm) (0.18–0.28) for the susceptible strain ( SUS ) and 210.6 µg mL −1 (175.90–258.10) for the lufenuron‐resistant strain ( LUF ‐R), based on diet‐overlay bioassay. The resistance ratio was ≈ 915‐fold. The LC 50 values for reciprocal crosses were 4.89 µg mL −1 (3.79–5.97) for female LUF ‐R and male SUS and 5.74 µg mL −1 (4.70–6.91) for female SUS and male LUF ‐R, indicating that the inheritance of S. frugiperda resistance to lufenuron is an autosomal, incompletely recessive trait. Backcrosses of the progeny of reciprocal crosses with the parental LUF ‐R showed a polygenic effect. The estimated minimum number of independent segregations was in the 11.02 range, indicating that resistance to lufenuron is associated with multiple genes in S. frugiperda . CONCLUSIONS Based on genetic crosses, the inheritance pattern of lufenuron resistance in S. frugiperda was autosomal, incompletely recessive and polygenic. Implications of this finding to IRM are discussed in this paper. © 2015 Society of Chemical Industry