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Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods
Author(s) -
GomezPolo Priscila,
Alomar Oscar,
Castañé Cristina,
AznarFernández Thaïs,
Lundgren Jonathan G,
Piñol Josep,
Agustí Nuria
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3989
Subject(s) - anthocoridae , biology , thripidae , predation , intraguild predation , trophic level , aphididae , hemiptera , biological pest control , ecology , botany , pest analysis , predator , homoptera
BACKGROUND The aphid Nasonovia ribisnigri (Mosley) (Hemiptera: Aphididae) and the thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) are common pests in Mediterranean lettuce crops, where Orius spp. are common generalist predators. Predation by Orius spp. was studied in a lettuce plot by conventional polymerase chain reaction ( PCR ) and real‐time PCR analyses using specific primers of both main pests. Also, high‐throughput sequencing was used to have a wider approach of the diet of these predators in natural field conditions. RESULTS Molecular analyses indicated a higher predation on N. ribisnigri in spring and on F. occidentalis in summer. Predation on alternative prey, like Collembola, was also found in both seasons. Real‐time PCR was more sensitive than conventional PCR in showing the target trophic links, whereas high‐throughput sequencing revealed predation on other natural enemies – intraguild predation ( IGP ), showing other trophic interactions of Orius majusculus within the studied ecosystem. CONCLUSIONS This study gives important information about the trophic relationships present in Mediterranean lettuce crops in different periods of the year. The detected predation by Orius spp. on alternative prey, as well as on other natural enemies, should be further investigated to clarify whether it adds or detracts to the biological control of N. ribisnigri and F. occidentalis . © 2015 Society of Chemical Industry