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Insight into the Meligethes aeneus voltage‐sensitive sodium channel structure and an attempt to select the best pyrethroid ligands
Author(s) -
ObrępalskaStęplowska Aleksandra,
Czerwoniec Anna,
Wieczorek Przemysław,
Wrzesińska Barbara
Publication year - 2016
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3984
Subject(s) - sodium channel , biology , pyrethroid , in silico , docking (animal) , deltamethrin , genetics , ecology , chemistry , pesticide , gene , sodium , medicine , nursing , organic chemistry
Abstract BACKGROUND The voltage‐sensitive sodium channel ( VSSC ) is a target for the pharmacological action of pyrethroids which are used in controlling pests, including those of agricultural importance. Among these is the pollen beetle ( Meligethes aeneus F.) – the most serious pest of Brassica napus . Owing to the heavy use of pyrethroids, a widespread build‐up of resistance has occurred. The main cause of pyrethroid insensitivity in M. aeneus is considered to be an increased oxidative metabolism; however, the additional mechanism of resistance associated with mutations in the VSSC might contribute to this phenomenon. RESULTS We generated a VSSC 3D model to study the docking affinities of pyrethroids to their target site within the channel. Our goal was to identify the pyrethroids for which docking affinity scores were high and not affected by potential mutations in the VSSC . We found that the docking scores of cypermethrin are hardly influenced by the appearance of point mutations. Additionally, tau‐fluvalinate, deltamethrin and bifenthrin are VSSC ligands with high affinity scores. CONCLUSIONS Our docking models suggest that point mutations in the VSSC binding pocket might affect the stability of ligand interactions and change the pattern of ligand docking locations, which might have a potential effect on VSSC gating properties. © 2015 Society of Chemical Industry