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Identification and characterisation of multiple glutathione S ‐transferase genes from the diamondback moth, Plutella xylostella
Author(s) -
Chen Xi'en,
Zhang Yalin
Publication year - 2015
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3884
Subject(s) - diamondback moth , plutella , biology , indoxacarb , glutathione s transferase , glutathione , gene , gene expression , midgut , microbiology and biotechnology , genetics , enzyme , botany , biochemistry , larva , pesticide , agronomy
BACKGROUND The diamondback moth ( DBM ), Plutella xylostella , is one of the most harmful insect pests on crucifer crops worldwide. In this study, 19 cDNAs encoding glutathione S ‐transferases ( GSTs ) were identified from the genomic and transcriptomic database for DBM ( KONAGAbase ) and further characterized. RESULTS Phylogenetic analysis showed that the 19 GSTs were classified into six different cytosolic classes, including four in delta, six in epsilon, three in omega, two in sigma, one in theta and one in zeta. Two GSTs were unclassified. RT‐PCR analysis revealed that most GST genes were expressed in all developmental stages, with higher expression in the larval stages. Six DBM GSTs were expressed at the highest levels in the midgut tissue. Twelve purified recombinant GSTs showed varied enzymatic properties towards 1‐chloro‐2,4‐dinitrobenzene and glutathione, whereas rPxGSTo2 , rPxGSTz1 and rPxGSTu2 had no activity. Real‐time quantitative PCR revealed that expression levels of the 19 DBM GST genes were varied and changed after exposure to acephate, indoxacarb, beta‐cypermethrin and spinosad. PxGSTd3 was significantly overexpressed, while PxGSTe3 and PxGSTs2 were significantly downregulated by all four insecticide exposures. CONCLUSION The changes in DBM GST gene expression levels exposed to different insecticides indicate that they may play individual roles in tolerance to insecticides and xenobiotics. © 2014 Society of Chemical Industry