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Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi‐quantitative AS‐PCR assay
Author(s) -
Hashimoto Maki,
Aoki Yoshinao,
Saito Seiya,
Suzuki Shunji
Publication year - 2015
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3867
Subject(s) - botrytis cinerea , biology , gene , genetics , microbiology and biotechnology , heteroplasmy , polymerase chain reaction , botany , mutation
BACKGROUND An in‐depth understanding of quinone outside inhibitor ( QoI )‐fungicide‐resistant Botrytis cinerea isolates in a vineyard is expected to contribute to the development of an optimum disease management programme for the control of grape grey mould. RESULTS The resistance and structure of the cytochrome b gene in B. cinerea collected from a Japanese vineyard were characterised. The semi‐quantitative allele‐specific primer–polymerase chain reaction ( AS‐PCR ) assay developed in the present study was able to distinguish heteroplasmic status from homoplasmic status at codon 143 of the cytochrome b gene in QoI ‐fungicide‐resistant B. cinerea from vineyards in Japan. With this assay it was demonstrated that the repeated introduction of QoI fungicide selection pressure increased the ratio of G143A ‐mutated cytochrome b genes in B. cinerea isolates. CONCLUSION It is proposed that the semi‐quantitative AS‐PCR assay is a reliable tool for the detection of QoI fungicide resistance and the evaluation of homoplasmic/heteroplasmic status at codon 143 of the cytochrome b gene in B. cinerea isolates. © 2014 Society of Chemical Industry