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Nuclear and cytoplasmic differentiation among Mediterranean populations of Bemisia tabaci : testing the biological relevance of cytotypes
Author(s) -
Terraz Gabriel,
Gueguen Gwenaelle,
Arnó Judit,
Fleury Frédéric,
Mouton Laurence
Publication year - 2014
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3792
Subject(s) - allopatric speciation , biology , sympatry , species complex , sympatric speciation , microsatellite , genetic diversity , evolutionary biology , zoology , ecology , phylogenetic tree , genetics , population , allele , demography , sociology , gene
BACKGROUND The taxonomy of the species complex Bemisia tabaci is still an unresolved issue. Recently, phylogenetic analysis based on mtCOI identified 31 cryptic species. However, mitochondrial diversity is observed within these species, associated with distinct symbiotic bacterial communities forming associations, which here are called cytotypes. The authors investigated the biological significance of two cytotypes ( Q1 and Q2 ) belonging to the Mediterranean species, which have only been found in allopatry in the Western Mediterranean to date. Sampling was done over a few years in Western Europe, and sympatric situations were found that allowed their reproductive compatibility to be tested in the field with the use of microsatellites. RESULTS The field survey indicated that, in spite of its recent introduction, Q2 is well established in France and Spain, where it coexists with Q1 . Microsatellite data showed that, in allopatry, Q1 and Q2 are highly differentiated, while there is little or no genetic differentiation when they coexist in sympatry, suggesting a high rate of hybridisation. Crossing experiments in the lab confirmed their interfertility. CONCLUSION Q1 and Q2 hybridise, which confirms that they belong to the same species, in spite of the high degree of genetic differentiation at both the cytoplasmic and nuclear levels, and also suggests that their symbiotic bacteria do not prevent hybridisation. © 2014 Society of Chemical Industry