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Detection of the I1781L mutation in fenoxaprop‐ p ‐ethyl‐resistant American sloughgrass ( Beckmannia syzigachne Steud.), based on the loop‐mediated isothermal amplification method
Author(s) -
Pan Lang,
Li Jun,
Zhang Wenna,
Dong Liyao
Publication year - 2015
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3777
Subject(s) - loop mediated isothermal amplification , biology , mutation , amino acid substitution , genetics , substitution (logic) , microbiology and biotechnology , isoleucine , leucine , dna , gene , amino acid , computer science , programming language
BACKGROUND The increasing use of fenoxaprop‐ p ‐ethyl has resulted in evolved resistance in American sloughgrass ( Beckmannia syzigachne Steud.). Target‐site‐based resistance to acetyl‐ CoA carboxylase ( ACCase ) inhibitors in B. syzigachne occurs owing to an isoleucine‐to‐leucine substitution at residue 1781 ( I1781L ) of the ACCase enzyme. A rapid detection method is needed to identify the resistance‐conferring substitution. RESULTS Four populations of B. syzigachne that were resistant to fenoxaprop‐ p ‐ethyl and contained the I1781L substitution were identified. Conventional PCR and derived cleaved amplified polymorphic sequence ( dCAPS ) methods were used to detect the mutation. Additionally, a rapid nucleic acid detection method, loop‐mediated isothermal amplification ( LAMP ), was successfully developed and used to detect the genetic mutation underlying the I1781L substitution in the B. syzigachne ACCase enzyme. CONCLUSION This report is the first to describe the application of a LAMP assay for mutation detection in herbicide‐resistant weeds. The assay does not require specialised equipment: only a standard laboratory bath is needed. This technique could be employed for detecting the I1781L substitution in B. syzigachne plants and seeds. © 2014 Society of Chemical Industry