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Oxidative stress response of Beauveria bassiana to Bordeaux mixture and its influence on fungus growth and development
Author(s) -
Martins Fátima,
Pereira José Alberto,
Baptista Paula
Publication year - 2014
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3675
Subject(s) - beauveria bassiana , entomopathogenic fungus , catalase , bassiana , laccase , fungicide , spore germination , superoxide dismutase , germination , mycelium , fungus , spore , oxidative stress , biology , food science , horticulture , botany , enzyme , biological pest control , biochemistry
BACKGROUND Chemical fungicides used to manage plant diseases may negatively affect beneficial fungi such as entomopathogens. In this study, the participation of the antioxidative system in the entomopathogenic fungus Beauveria bassiana exposed to the copper‐based Bordeaux mixture fungicide and its relation with fungus growth and development were examined. The fungus was grown in submerged culture containing Bordeaux mixture at the recommended dose. Within the first 24 h of elicitation, the vegetative growth, germination, sporulation and activity of amylase and laccase and the antioxidative enzymes catalase ( CAT ) and superoxide dismutase ( SOD ), as well as the production of hydrogen peroxide ( H 2 O 2 ), were evaluated . RESULTS Bordeaux mixture inhibited B. bassiana germination (between 65 and 88%) and sporulation (between 15 and 57%) and significantly increased laccase production (≥130%), especially within the first 4 h of fungus exposure. By contrast, the mycelial growth was found to be less affected by the fungicide. These effects were accompanied with a significant increase in H 2 O 2 levels in fungal cells, as well as in SOD activity, but not in CAT , showing clear signs of increased oxidative stress . CONCLUSION The effect of Bordeaux mixture on B. bassiana development was probably due to the toxicity of the copper ion itself, and it also induced an oxidative state in fungal cells. © 2013 Society of Chemical Industry