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Identification of an alternative knockdown resistance ( kdr )‐like mutation, M918L , and a novel mutation, V1010A , in the Thrips tabaci voltage‐gated sodium channel gene
Author(s) -
Wu Meixiang,
Gotoh Hiroki,
Waters Timothy,
Walsh Douglas B,
Lavine Laura Corley
Publication year - 2014
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3638
Subject(s) - biology , knockdown resistance , genetics , pyrethroid , thrips , mutation , gene knockdown , sodium channel , western flower thrips , gene , pest analysis , microbiology and biotechnology , botany , thripidae , pesticide , sodium , chemistry , organic chemistry , cyfluthrin , agronomy
BACKGROUND Knockdown resistance ( kdr ) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci . To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations . RESULTS Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda‐cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage‐gated sodium channel gene. One mutation resulted in the non‐synonymous substitution of methionine with leucine ( M918L ), which is well known to be responsible for super knockdown resistance in some pest species. Another non‐synonymous substitution, a valine ( GTT ) to alanine ( GCT ) replacement at amino acid 1010 ( V1010A ) was identified in our study and was associated with lambda‐cyhalothrin resistance . CONCLUSION We have characterized a known kdr mutation and identified a novel mutation in the voltage‐gated sodium channel gene of Thrips tabaci associated with resistance to lambda‐cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry