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Camptothecin‐induced expression of programmed cell death gene 11 in Spodoptera litura
Author(s) -
Gong Liang,
Wang Huidong,
Huang Jingfei,
Hu Meiying,
Hu Zheng,
Zhong Guohua
Publication year - 2014
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3588
Subject(s) - camptothecin , spodoptera litura , biology , tunel assay , midgut , apoptosis , microbiology and biotechnology , terminal deoxynucleotidyl transferase , programmed cell death , biochemistry , botany , larva
BACKGROUND Camptothecin, one of the main active components of extract from the bark of the Chinese camptotheca tree, has been reported as a potent insecticide against various insect species. However, the mechanism of action of camptothecin as a botanical pesticide is not completely understood . RESULTS In this study, the full‐length cDNA ( GeneBank number JF681972 ) of Spodoptera litura programmed cell death protein 11 ( pcdp 11 ) isoform 1 was cloned, sequenced and characterised. Quantitative real‐time PCR ( qRT‐PCR ) data showed that pcdp 11 was differentially expressed during the developmental stages, with significantly high expression during the transition from egg to larva and larva to pupa. Furthermore, pcdp 11 was upregulated in a time‐dependent manner in SL ‐1 cells after treatment with 1.2 µg mL −1 of camptothecin. The induced expression profile of pcdp 11 in the larval midgut after feeding camptothecin was visualised by fluorescence in situ hybridisation ( FISH ) and further quantified by qRT‐PCR . Apoptosis in camptothecin‐treated larval midguts was confirmed using terminal deoxynucleotidyl transferase dUTP nick end labelling ( TUNEL ) staining . CONCLUSION The results reveal a clear functional link between pcdp 11 expression and camptothecin‐induced apoptosis, and prove that camptothecin exhibits strong toxicity towards S. litura by inducing midgut epithelial cell apoptosis. © 2013 Society of Chemical Industry