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Integrated biological and chemical control of rice sheath blight by Bacillus subtilis NJ ‐18 and jinggangmycin
Author(s) -
Peng Di,
Li Shandong,
Wang Jianxin,
Chen Changjun,
Zhou Mingguo
Publication year - 2014
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3551
Subject(s) - rhizoctonia solani , sheath blight , bacillus subtilis , fungicide , biological pest control , rhizoctonia , biology , agronomy , disease control , horticulture , bacteria , microbiology and biotechnology , genetics
BACKGROUND Sheath blight caused by Rhizoctonia solani Kühn is a major disease of rice that greatly reduces yield and grain quality and jinggangmycin is the most widely used fungicide to control this disease in China. Bacillus subtilis NJ ‐18 has broad antimicrobial activity to many phytopathogenic bacteria and fungi; it is especially effective against Rhizoctonia solani . Laboratory, greenhouse and field tests were conducted to determine the effect of combining the biological control agent Bacillus subtilis NJ ‐18 with the fungicide jinggangmycin for control of rice sheath blight.RESULTS Growth of NJ ‐18 in vitro was not affected by jinggangmycin. In a greenhouse experiment, disease control was greater with a mixture of NJ ‐18 and jinggangmycin than with either alone; a mixture of NJ ‐18 at 10 8 cfu mL −1 and jinggangmycin at 50 or 100 mg L −1 reduced lesion length by 35% and 20%, respectively, and the combinations showed a synergistic action. In three field trials, disease control was significantly greater with a mixture of NJ ‐18 at 10 8 cfu mL −1 and jinggangmycin at 75 or 150 g a.i. ha −1 than with either component alone.CONCLUSION The results of the study indicate that, when Bacillus subtilis NJ ‐18 strain was combined with jinggangmycin, there was an increased suppression of rice sheath blight, and thus could provide an alternative disease control option. © 2013 Society of Chemical Industry