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Involvement of superoxide dismutase in oxidative stress in the oriental fruit fly, Bactrocera dorsalis : molecular cloning and expression profiles
Author(s) -
Gao XueMeng,
Jia FuXian,
Shen GuangMao,
Jiang HuaQin,
Dou Wei,
Wang JinJun
Publication year - 2013
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3503
Subject(s) - bactrocera dorsalis , superoxide dismutase , oxidative stress , biology , reactive oxygen species , antioxidant , microbiology and biotechnology , biochemistry , pest analysis , botany , tephritidae
Background Bactrocera dorsalis , one of the most economically important fruit fly pests in East Asia, is well adapted to various environmental conditions. Pesticides, pathogens and other stresses can cause oxidative damage in most organisms. The superoxide dismutase ( SOD ) family contains some of the most important enzymes in the antioxidant protection system of the fruit fly and other organisms . Results Four full‐length cDNA sequences encoding one MnSOD ( BdSOD2 ‐1 ) and three Cu– ZnSODs ( BdSOD1 ‐1 , BdSOD1 ‐2 and BdSOD1 ‐3 ) were cloned. The expression profiles of these four genes under different stresses showed them to be involved in response to detrimental conditions including heavy metals, pesticides, extreme temperatures and lipopolysaccharide ( LPS ) stresses. More specifically, the expression levels of these genes were found to be depressed in the presence of copper, zinc and manganese. The expression of all four SOD genes increased upon exposure to lead, cadmium, low temperature (0 °C) and LPS stresses. Only BdSOD1 ‐3 transcription increased significantly at high temperature (40 °C) exposure. The expressions levels of BdSOD1 ‐2 and BdSOD1 ‐3 increased significantly in the presence of β ‐cypermethrin and malathion, but only the expression of BdSOD2 ‐1 increased in the presence of avermectin treatment . Conclusion These different expression profiles suggest that the four BdSODs play different roles and respond to different oxidative stresses in B. dorsalis . Some BdSODs undergo specific reaction in the response to specific oxidative stresses. © 2013 Society of Chemical Industry