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A novel Cph‐like gene involved in histogenesis and maintenance of midgut in Bombyx mori
Author(s) -
Gan Liping,
Zhuo Weiwei,
Li Juan,
Wang Ying,
Sima Yanghu,
Xu Shiqing
Publication year - 2013
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3501
Subject(s) - midgut , biology , bombyx mori , gene , gene knockdown , gene expression , helicoverpa armigera , complementary dna , microbiology and biotechnology , rna interference , genetics , larva , rna , botany
BACKGROUND Male‐biased silkworm larva resistance is useful for sericulture and lepidopteran pest control. According to previous research, the mechanism underlying this resistance might be related to midgut‐specific proteins . RESULTS A midgut‐specific and novel hypothetical cuticular protein‐like (Cph‐like) gene was cloned, based on sex‐disparity serial analysis of gene expression (SAGE) libraries of the B. mori midgut. The complete cDNA contained 676 bp and encoded 165 amino acid residues. The gene was located on chromosome 19 and it had only one short 75 bp intron. The Cph‐like expression level was downregulated by exogenous 20‐hydroxyecdysone or starvation, but upregulated by exogenous methoprene or food intake. Knockdown (siRNA) of the Cph‐like gene suppressed the appetite and delayed larval development, while it also degraded enterocytes and damaged the midgut morphology. Furthermore, the male‐biased cytoplasmic polyhedrosis virus (BmCPV) resistance of larvae was decreased . Conclusion The Cph‐like gene is a midgut‐specific novel gene in B. mori that may participate in histogenesis and midgut maintenance. © 2013 Society of Chemical Industry