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Functional analysis of the β 2 ‐tubulin gene of Fusarium graminearum and the β ‐tubulin gene of Botrytis cinerea by homologous replacement
Author(s) -
Liu ShengMing,
Duan YaBing,
Ge ChangYan,
Chen ChangJun,
Zhou MingGuo
Publication year - 2013
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3474
Subject(s) - botrytis cinerea , carbendazim , biology , mutant , tubulin , gene , fungicide , gibberella zeae , fusarium , fungi imperfecti , conidiation , genetics , microbiology and biotechnology , botany , microtubule
Background Resistance to carbendazim and other benzimidazole fungicides in Botrytis cinerea and most other fungi is usually conferred by one or several allelic mutations in the β ‐tubulin. Carbendazim resistance in Fusarium graminearum , however, differs from that in B. cinerea and other fungi in that F. graminearum has two β ‐tubulins (Fgtub1 and Fgtub2) rather than one, and the resistance is conferred by mutations in the β 2 ‐tubulin. In a previous study, the β 1 ‐tubulin of F. graminearum (Fgtub1) was replaced with the β ‐tubulin of B. cinerea conferring carbendazim resistance ( BctubE198A ). The transformants were sensitive to carbendazim . Results BctubE198A was successfully transferred into the β 2 ‐tubulin locus of F. graminearum (Fgtub2) via homologous replacement. The mutants were still sensitive to carbendazim. Furthermore, Fgtub2 of the mutant 20C1 (Fgtub1 had been replaced with BctubE198A ) and Fgtub1 of the mutant 20D10 (Fgtub2 had been replaced with BctubE198A ) were deleted. Surprisingly, the mutants were also sensitive to carbendazim. Meanwhile, the biological characteristics of all the mutants were determined . Conclusion The B. cinerea β ‐tubulin (Bctub) could complement the function of the two F. graminearum β ‐tubulins even when both were deleted. Expression of the β ‐tubulin conferring carbendazim resistance differs between pathogenic fungi. © 2012 Society of Chemical Industry