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Effect of trehalose on the biocontrol efficacy of Pichia caribbica against post‐harvest grey mould and blue mould decay of apples
Author(s) -
Zhao Lina,
Zhang Hongyin,
Lin Hetong,
Zhang Xiaoyun,
Ren Xiaofeng
Publication year - 2013
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.3463
Subject(s) - trehalose , botrytis cinerea , peroxidase , food science , biology , yeast , population , glycerol , nutrient , botrytis , horticulture , yeast extract , botany , chemistry , biochemistry , fermentation , enzyme , demography , sociology , ecology
Background The influence of adding trehalose to the culture medium on the efficacy of Pichia caribbica ( JSU ‐1) in controlling post‐harvest grey mould and blue mould decay of apples and the possible mechanisms involved were investigated . Results The antagonistic activity of P. caribbica harvested from nutrient yeast dextrose broth ( NYDB ) amended with trehalose at 5 mg mL −1 to post‐harvest grey mould and blue mould decay was improved greatly compared with that without trehalose. The intracellular trehalose content of P. caribbica harvested from NYDB amended with trehalose at 5 mg mL −1 was higher than that of P. caribbica harvested from NYDB . The population of P. caribbica could rapidly colonise and grow in apple wounds. Moreover, the application of P. caribbica cultivated in the culture medium of the trehalose supplement (5 mg mL −1 ) induced higher polyphenoloxidase ( PPO ) and peroxidase ( POD ) activity of apples compared with that cultivated by P. caribbica in the NYDB . SEM results suggested that P. caribbica harvested from NYDB amended with trehalose at 5 mg mL −1 showed stronger inhibition efficacy to Botrytis cinerea than P. caribbica harvested from NYDB . Conclusion The antagonistic activity of P. caribbica can be enhanced by adding trehalose to the medium. © 2012 Society of Chemical Industry