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Inheritance of resistance to Bacillus thuringiensis Cry1Ac toxin in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from India
Author(s) -
Kaur Paramjit,
Dilawari Vinod Kumar
Publication year - 2011
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.2185
Subject(s) - cry1ac , helicoverpa armigera , bacillus thuringiensis , biology , noctuidae , biopesticide , bt cotton , lepidoptera genitalia , veterinary medicine , spinosad , toxicology , rapd , microbiology and biotechnology , pest analysis , genetically modified crops , pesticide , horticulture , botany , transgene , agronomy , bacteria , gene , population , genetics , genetic diversity , demography , sociology , medicine
Abstract BACKGROUND: The cotton bollworm, Helicoverpa armigera (Hübner), is one of the most serious insect pests of cotton. It has developed resistance to almost all groups of chemical insecticides because of their intensive use. The failure of insecticides to control H. armigera has been a strong incentive for the adoption of transgenic cotton ( Bt cotton). However, the value of Bt could be diminished by widespread resistance development to Bt toxins in insect populations. Therefore, understanding the genetic basis of resistance is essential for developing and implementing strategies to delay and monitor resistance. RESULTS: A resistant strain designated as BM‐R was obtained from the cross of adults from Bathinda () and Muktsar (), Punjab, India, which showed the highest survival (60.68%) and LC 50 value (1.396 µg mL −1 diet). Similarly, a laboratory‐maintained strain from Hoshiarpur, Punjab, showed maximum susceptibility to Cry1Ac toxin with the lowest LC 50 value (0.087 µg mL −1 ), and was designated as HP‐S. The genetic purity of both strains was confirmed by RAPD profile analysis at each generation, and genetic similarity reached more than 90% after the third generation. Continuous maintenance of the resistant BM‐R strain on Cry1Ac resulted in an increase in LC 50 from 0.531 µg mL −1 in F 0 to 4.28 µg mL −1 in F 14 and 7.493 µg mL −1 in F 19 , while the LC 50 values for HP‐S larvae on diet without Cry1Ac increased to 0.106 and 0.104 µg mL −1 , which lay within the fiducial limits of the baseline LC 50 value. The mode of inheritance of resistance was elucidated through bioassay response of resistant, susceptible heterozygotes and backcross progeny to Cry1Ac incorporated in semi‐synthetic diet. CONCLUSION: Based on dominance, degree of dominance and backcross values, resistance was inferred to be polygenic, autosomal and inherited as a recessive trait. Copyright © 2011 Society of Chemical Industry