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Characterisation and toxicity of Bacillus thuringiensis strains from hazelnut pests and fields
Author(s) -
Sezen Kazim,
Kati Hatice,
Muratoglu Hacer,
Demirbag Zihni
Publication year - 2010
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1905
Subject(s) - bacillus thuringiensis , biology , bacillales , lepidoptera genitalia , biopesticide , bacillaceae , biological pest control , cereus , bacillus cereus , microbiology and biotechnology , botany , pesticide , bacteria , agronomy , genetics , bacillus subtilis
BACKGROUND: In order to find and identify more toxic insecticidal Bacillus thuringiensis Berliner ( Bt ) strains, a survey was carried out of B. thuringiensis isolate pests belonging to Coleoptera, Lepidoptera and Diptera and from soils in hazelnut fields. Of 16 isolates having Bacillus cereus ‐ B. thuringiensis morphology, eight were classified as B. thuringiensis because of the production of parasporal δ‐endotoxin crystals. RESULTS: In this study, eight isolates of B. thuringiensis from hazelnut pests (isolates Bn1, Mm2, Mnd and Xd3) and from hazelnut soils (isolates 6, 27, 40 and 46) have been characterised in detail. These isolates were compared with reference strains by electron microscopy, SDS‐PAGE analysis, cry gene content, serological test and insecticidal activity. CONCLUSION: Results indicate that Bn1 and MnD are B. thuringiensis subsp. kurstaki , and Mm2 and Xd3 are B. thuringiensis subsp. tenebrionis . In addition, isolate 6 is B. thuringiensis subsp. israelensis , isolates 27 and 46 are B. thuringiensis subsp. kumamotoensis and isolate 40 is B. thuringiensis subsp. indiana . The four B. thuringiensis isolates from hazelnut pests may be valuable as biological control agents against coleopteran and lepidopteran insects. Copyright © 2009 Society of Chemical Industry