Rapid method for detecting resistance to a QoI fungicide in  Plasmopara viticola  populations | Zendy

Furuya Seiichi | Zendy; Suzuki Shunji | Zendy; Kobayashi Hironori | Zendy; Saito Seiya | Zendy; Takayanagi Tsutomu | Zendy

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Rapid method for detecting resistance to a QoI fungicide in Plasmopara viticola populations

Author(s) -

Furuya Seiichi,

Suzuki Shunji,

Kobayashi Hironori,

Saito Seiya,

Takayanagi Tsutomu

Publication year - 2009

Publication title -

pest management science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.296

H-Index - 125

eISSN - 1526-4998

pISSN - 1526-498X

DOI - 10.1002/ps.1765

Subject(s) - plasmopara viticola , fungicide , downy mildew , azoxystrobin , biology , botany

BACKGROUND: The increasing occurrence of Qo inhibitor (QoI)‐fungicide‐resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR‐RFLP method. With this method, a glycine‐to‐alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan. CONCLUSION: It is proposed that the nested PCR‐RFLP method is a high‐speed, sensitive and reliable tool for detecting azoxystrobin‐resistant P. viticola populations. Copyright © 2009 Society of Chemical Industry

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