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A novel assay reveals the blockade of esterases by piperonyl butoxide
Author(s) -
Khot Anna C,
Bingham Georgina,
Field Linda M,
Moores Graham D
Publication year - 2008
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1603
Subject(s) - piperonyl butoxide , esterase , myzus persicae , chemistry , blockade , in vitro , in vitro toxicology , bioassay , toxicology , biochemistry , pharmacology , enzyme , biology , toxicity , botany , genetics , organic chemistry , receptor , aphid
BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase‐mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new ‘esterase interference’ assay detects the blockade of resistance‐associated esterases in a species, Myzus persicae Sülzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high‐throughput screening method for other potential synergists. Copyright © 2008 Society of Chemical Industry