A novel assay reveals the blockade of esterases by piperonyl butoxide | Zendy

Khot Anna C | Zendy; Bingham Georgina | Zendy; Field Linda M | Zendy; Moores Graham D | Zendy

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A novel assay reveals the blockade of esterases by piperonyl butoxide

Author(s) -

Khot Anna C,

Bingham Georgina,

Field Linda M,

Moores Graham D

Publication year - 2008

Publication title -

pest management science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.296

H-Index - 125

eISSN - 1526-4998

pISSN - 1526-498X

DOI - 10.1002/ps.1603

Subject(s) - piperonyl butoxide , esterase , myzus persicae , chemistry , blockade , in vitro , in vitro toxicology , bioassay , toxicology , biochemistry , pharmacology , enzyme , biology , toxicity , botany , genetics , organic chemistry , receptor , aphid

BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase‐mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new ‘esterase interference’ assay detects the blockade of resistance‐associated esterases in a species, Myzus persicae Sülzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high‐throughput screening method for other potential synergists. Copyright © 2008 Society of Chemical Industry

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