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Toxicity of dimethoate and fenoxycarb to honey bee brood ( Apis mellifera ), using a new in vitro standardized feeding method
Author(s) -
Aupinel Pierrick,
Fortini Dominique,
Michaud Bruno,
Marolleau Franck,
Tasei JeanNoël,
Odoux JeanFrançois
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1446
Subject(s) - dimethoate , brood , larva , biology , honey bee , toxicology , pesticide , toxicity , pupa , zoology , botany , ecology , medicine
A new in vitro method was devised to assess the effects of pesticides on honey bee brood. The method allowed the quantification of doses ingested by larvae and the assessment of larval and pupal mortality. Larval mortality in control samples was lower than 10%. Two active substances were tested: dimethoate and fenoxycarb. The LD 50 of dimethoate was 1.9 µg larva −1 48 h after oral exposure of larvae at day 4. Additional dose‐related effects on pupal mortality were noted. After a chronic intoxication, the NOAEC (No Observed Adverse Effect Concentration) for larval mortality at day 7 was 2.5 mg kg −1 , whereas a NOAEC of 5 mg kg −1 was found at day 22 for delayed effects on the reduction of adult emergence. Fenoxycarb applied at day 4 showed no effect on larvae, whereas emergence of adults was affected at doses higher than 6 ng larva −1 . Copyright © 2007 Society of Chemical Industry