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Isolation and antifungal activity of lignans from Myristica fragrans against various plant pathogenic fungi
Author(s) -
Cho Jun Young,
Choi Gyung Ja,
Son Seung Wan,
Jang Kyoung Soo,
Lim He Kyoung,
Lee Sun Og,
Sung Nack Do,
Cho Kwang Yun,
Kim JinCheol
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1420
Subject(s) - biology , myristica fragrans , magnaporthe grisea , alternaria alternata , alternaria , botany , spore germination , microbiology and biotechnology , germination , oryza sativa , biochemistry , gene
BACKGROUND: In a search for plant extracts with potent in vivo antifungal activity against various plant diseases, we found that treatment with a methanol extract of Myristica fragrans Houttyn (nutmeg) seeds reduced the development of various plant diseases. The objectives of the present study were to isolate and determine antifungal substances from My. fragrans and to evaluate their antifungal activities. RESULTS: Three antifungal lignans were isolated from the methanol extract of My. fragrans seeds and identified as erythro ‐austrobailignan‐6 (EA6), meso ‐dihydroguaiaretic acid (MDA) and nectandrin‐B (NB). In vitro antimicrobial activity of the three lignans varied according to compound and target species. Alternaria alternata, Colletotrichum coccodes, C. gloeosporioides, Magnaporthe grisea, Agrobacterium tumefaciens, Acidovorax konjaci and Burkholderia glumae were relatively sensitive to the three lignans. In vivo , all three compounds effectively suppressed the development of rice blast and wheat leaf rust. In addition, EA6 and NB were highly active against the development of barley powdery mildew and tomato late blight, respectively. Both MDA and NB also moderately inhibited the development of rice sheath blight. CONCLUSION: This is the first study to demonstrate the in vitro and in vivo antifungal activities of the three lignans from My. fragrans against plant pathogenic fungi. Copyright © 2007 Society of Chemical Industry