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Impact of a Turkish isolate and a plaque‐purified variant of SpliNPV‐B on larval stage development of Spodoptera littoralis (Lepidoptera: Noctuidae) Boisd
Author(s) -
Toprak Umut,
Gürkan M Oktay,
Bayram Şerife
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1370
Subject(s) - spodoptera littoralis , noctuidae , biology , instar , lepidoptera genitalia , larva , biological pest control , inoculation , spodoptera , botany , horticulture , biochemistry , gene , recombinant dna
Baculoviruses can alter the development and physiological status of their insect hosts. In the present study, two Spodoptera littoralis nucleopolyhedroviruses (SpliNPV‐TR1 and SpliNPV‐M2) were examined in terms of their effect on the stage development of S. littoralis at two doses/concentrations by inoculation of neonates or third instars. Both isolates had an acute pathological effect on neonates. However, larval development was prolonged (6–36 h) by either infection when neonates were infected. When third‐instar larvae were inoculated, no mortality occurred until insects moulted to later stages. When the isolates were compared, more mortality occurred in the fifth instar with SpliNPV‐M2 than with SpliNPV‐TR1, whereas less mortality occurred in the sixth instar with SpliNPV‐M2 than with SpliNPV‐TR1. However, none of the isolates demonstrated a dose effect. Larvae died at 108–198 h post‐inoculation at all infections, and none of them pupated. Larval development was retarded (0–126 h) for each stage when third‐instar larvae were inoculated. Consequently, SpliNPV‐M2 would be a more effective control agent than SpliNPV‐TR1 for control of S. littoralis since it kills larvae at earlier developmental stages. Copyright © 2007 Society of Chemical Industry