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Impact of a new biopesticide produced by Paenibacillus sp. strain B2 on the genetic structure and density of soil bacterial communities
Author(s) -
Selim Sameh,
MartinLaurent Fabrice,
Rouard Nadine,
Gianinazzi Silvio,
van Tuinen Diederik
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1335
Subject(s) - 16s ribosomal rna , biology , paenibacillus , denitrifying bacteria , biopesticide , bacteria , bacterial strain , colony forming unit , strain (injury) , polymerase chain reaction , bacterial colony , paenibacillus polymyxa , food science , bacterial growth , veterinary medicine , microbiology and biotechnology , pesticide , agronomy , gene , chemistry , genetics , medicine , organic chemistry , anatomy , nitrogen , denitrification
The effect of paenimyxin, a new biopesticide produced by Paenibacillus sp. strain B2, on the density of soil bacterial communities was assessed by colony counting and by 16S rDNA and nirK quantitative polymerase chain reaction (PCR). Paenimyxin had a negative effect on the bacterial colony‐forming unit (CFU) number, which was significantly reduced 2 and 4 days after treatment. The effect of paenimyxin on cultivatable bacteria was negligible 7 days after treatment. Approximately 10 7 16S rDNA sequences per gram of soil (dry weight) were detected by quantitative PCR in all samples. Paenimyxin did not affect the quantification of 16S rDNA or of the denitrifying bacterial community. In addition, RISA fingerprinting showed that the genetic structure of the bacterial communities was significantly modified 2 days after paenimyxin application at 50 µ M and 4 days after treatment at lower concentrations (0.5 and 5 µ M ). The impact of paenimyxin treatment on the genetic structure of soil bacterial communities was transient, as no effect could be observed after 7, 14 and 28 days when compared with the untreated control. Copyright © 2007 Society of Chemical Industry