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Isolation and characterization of a novel simazine‐degrading β‐proteobacterium and detection of genes encoding s ‐triazine‐degrading enzymes
Author(s) -
Iwasaki Akio,
Takagi Kazuhiro,
Yoshioka Yuichi,
Fujii Kunihiko,
Kojima Yuki,
Harada Naoki
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1334
Subject(s) - simazine , 16s ribosomal rna , biology , strain (injury) , gene , genbank , pseudomonas , microbiology and biotechnology , nucleic acid sequence , sequence analysis , bacteria , accession number (library science) , atrazine , genetics , anatomy , pesticide , agronomy
A moderately persistent herbicide, simazine, has been used globally and detected as a contaminant in soil and water. The authors have isolated a simazine‐degrading bacterium from a simazine‐degrading bacterial consortium that was enriched using charcoal as a microhabitat. The isolate, strain CDB21, was gram‐negative, rod‐shaped (0.5–0.6 µm × 1.0–1.2 µm) and motile by means of a single polar flagellum. Based on 16S rRNA sequence analysis, strain CDB21 was identified as a novel β‐proteobacterium exhibiting 100% sequence identity with the uncultured bacterium HOClCi25 (GenBank accession number AY328574). PCR using primers that were specific for the genes of the atrazine‐degrading enzymes ( atzABCDEF ) of Pseudomonas sp. strain ADP showed that strain CDB21 also possessed the entire set of genes of these enzymes. Nucleotide sequences of the atzCDEF genes of strain CDB21 were 100% identical to those of Pseudomonas sp. strain ADP. Sequence identity of the atzA genes between these bacteria was 99.7%. The 398‐nucleotide upstream fragment of the atzB gene of strain CDB21 was 100% identical to ORF30 of Pseudomonas sp. strain ADP, and the 1526‐nucleotide downstream fragment showed 99.8% sequence similarity to the atzB gene of the pseudomonad. Copyright © 2007 Society of Chemical Industry