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Esterase‐mediated bifenthrin resistance in a multiresistant strain of the two‐spotted spider mite, Tetranychus urticae
Author(s) -
Van Leeuwen Thomas,
Tirry Luc
Publication year - 2007
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1314
Subject(s) - bifenthrin , tetranychus urticae , esterase , strain (injury) , spider mite , biology , microbiology and biotechnology , food science , enzyme , biochemistry , pesticide , botany , mite , anatomy , agronomy
A field‐collected multiresistant strain of Tetranychus urticae Koch exhibiting high resistance to bifenthrin was investigated in comparison with a susceptible laboratory strain. The esterase inhibitor S , S , S ‐tributyl‐phosphorotrithioate (DEF) was able strongly to synergise bifenthrin toxicity in the resistant strain. Optimal conditions for determining esterase activities in T. urticae were determined, and a higher esterase activity towards several artificial substrates was found in this resistant strain, which had a preference for hydrolysing 4‐nitrophenyl butyrate. Bifenthrin was able to bind the active centres of T. urticae esterases in vitro , as was determined after competition experiments by a Dixon plot, revealing a higher affinity of bifenthrin in the resistant strain. Bifenthrin‐hydrolysing activity in the resistant and susceptible strains was examined in vitro and quantified with gas chromatography. A 7.2‐fold higher metabolising rate was found in the resistant strain. Copyright © 2006 Society of Chemical Industry