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Control of Aspergillus growth and aflatoxin production using natural maize phytochemicals under different conditions of water activity
Author(s) -
Nesci Andrea V,
Etcheverry Miriam G
Publication year - 2006
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1251
Subject(s) - aflatoxin , aspergillus flavus , chemistry , food science , ferulic acid
The effects of the natural phytochemicals trans ‐cinnamic acid (CA) and ferulic acid (FA) alone at concentrations of 1–25 m M and in 16 combinations (M: mixtures) on growth and aflatoxin B 1 production by Aspergillus flavus Link and A. parasiticus Speare were evaluated. Studies on growth rate and aflatoxin B 1 production were carried out in vitro in relation to a water activity a w of 0.999, 0.971, 0.955 and 0.937. Overall, CA at concentrations of 10 and 20 m M and FA–CA mixtures M3 (20 + 5 m M respectively), M8 (25 + 5 m M ), M9 (1 + 10 m M ), M10 (10 + 10 m M ), M11 (20 + 10 m M ), M12 (25 + 10 m M ), M13 (1 + 20 m M ), M14 (10 + 20 m M ), M15 (20 + 20 m M ) and M16 (25 + 20 m M ) were the treatments most effective at inhibiting growth of the four species assayed. All strains were much more sensitive to all natural phytochemicals tested on growth rate at a w = 0.937. CA and the FA–CA mixtures M1 (1 + 1 m M respectively), M4 (25 + 1 m M ), M5 (1 + 5 m M ), M6 (10 + 1 m M ), M7 (20 + 1 m M ), M8 (25 + 5 m M ), M9 (1 + 10 m M ), M10 (10 + 10 m M ), M11 (20 + 10 m M ), M12 (25 + 10 m M ), M13 (1 + 20 m M ), M14 (10 + 20 m M ), M15 (20 + 20 m M ) and M16 (25 + 20 m M ) completely inhibited aflatoxin B 1 production by all strains at a w = 0.999, 0.971, 0.955 and 0.937. Decreased aflatoxin B 1 levels in comparison with the control were observed with FA at 1, 10, 20 and 25 m M with the strains RCM89, RCM108 and RCM38 at a w = 0.971, 0.955 and 0.999 respectively. The data show that CA and FA can be considered as effective fungitoxicants for A. flavus and A. parasiticus in in vitro assay. The information obtained is part of an ongoing study to determine their application at the storage level. Copyright © 2006 Society of Chemical Industry