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Rapid bidirectional allele‐specific PCR identification for triazine resistance in higher plants
Author(s) -
Tian Xin,
Darmency Henri
Publication year - 2006
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1197
Subject(s) - polymerase chain reaction , genetics , allele , biology , gene , mutation , gene flow , identification (biology) , computational biology , botany , genetic variation
Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser 264 –Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele‐specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined. Copyright © 2006 Society of Chemical Industry