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Involvement of a sodium channel mutation in pyrethroid resistance in Cydia pomonella L, and development of a diagnostic test
Author(s) -
BrunBarale Alexandra,
Bouvier JeanCharles,
Pauron David,
Bergé JeanBaptiste,
Sauphanor Benoit
Publication year - 2005
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.1002
Subject(s) - knockdown resistance , codling moth , biology , deltamethrin , pyrethroid , tortricidae , sodium channel , pesticide resistance , genetics , cypermethrin , point mutation , mutation , pest analysis , lepidoptera genitalia , gene , botany , pesticide , sodium , chemistry , agronomy , organic chemistry , cyfluthrin
Populations of the codling moth, Cydia pomonella L (Lepidoptera, Tortricidae) have developed resistance to several classes of insecticide such as benzoylureas, juvenile hormone analogues, ecdysone agonists and pyrethroids, but the corresponding resistance mechanisms have not been extensively studied. Knockdown resistance ( kdr ) to pyrethroid insecticides has been associated with point mutations in the para sodium channel gene in a great variety of insect pest species. We have studied two susceptible strains (S and Sv) and two resistant strains (Rt and Rv) of C pomonella that exhibited 4‐ and 80‐fold resistance ratios to deltamethrin, respectively. The region of the voltage‐dependent sodium channel gene which includes the position where kdr and super‐ kdr mutations have been found in Musca domestica L was amplified. The kdr mutation, a leucine‐to‐phenylalanine replacement at position 1014, was found only in the Rv strain. In contrast, the super‐ kdr mutation, a methionine‐to‐threonine replacement at position 918, was not detected in any C pomonella strain. These data allowed us to develop a PCR‐based diagnostic test (PASA) to monitor the frequency of the kdr mutation in natural populations of C pomonella in order to define appropriate insecticide treatments in orchards. Copyright © 2005 Society of Chemical Industry