Open AccessEffects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)
Author(s) -
Ajilore Bamidele S.,
Alli Adetayo A.,
Oluwadairo Tolulope O.
Publication year - 2018
Publication title -
pharmacology research and perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.975
H-Index - 27
ISSN - 2052-1707
DOI - 10.1002/prp2.401
Subject(s) - chlorpyrifos , chemistry , cholinesterase , organophosphate , magnesium , acetylcholinesterase , enzyme , biochemistry , pharmacology , pesticide , biology , organic chemistry , agronomy
The present study investigated possible benefits of magnesium ion (as MgCl 2 ) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing EDTA were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl 2 . Acetylcholinesterase, Na + /K + ATP ase and Ca 2+ ATP ase activities were evaluated. Results showed that Chlorpyrifos significantly ( P < .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na + /K + ATP ase and Ca 2+ ATP ase were also significantly ( P < .05) reduced by chlorpyrifos while MgCl 2 counteracted effects of chlorpyrifos with significant ( P < .05) increase in the levels of cholinesterase, Na + /K + ATP ase and Ca 2+ ATP ase. We concluded that MgCl 2 neutralized effects of chlorpyrifos by promoting normal ATP ase activities and inhibiting release of acetylcholine from cell.