Open AccessMicroparticle of drug and nanoparticle: a biosynthetic route
Author(s) -
Sarkar Sounik,
Dasgupta Anjan Kr.
Publication year - 2015
Publication title -
pharmacology research and perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.975
H-Index - 27
ISSN - 2052-1707
DOI - 10.1002/prp2.188
Subject(s) - microparticle , nanotechnology , drug , context (archaeology) , drug delivery , confocal microscopy , hela , flow cytometry , nanoparticle , materials science , cytotoxicity , doxorubicin hydrochloride , drug carrier , doxorubicin , chemistry , biomedical engineering , pharmacology , medicine , cell , in vitro , chemical engineering , biochemistry , immunology , surgery , paleontology , chemotherapy , microbiology and biotechnology , engineering , biology
Microparticles ( MP s) have great potentiality in material science‐ based applications. Their use in biology is however limited to clinics and has rarely been exploited in the pharmaceutical context. Unlike nanoparticles (NPs), they are amenable to routine detection by flow cytometry and confocal microscopy. Though MP s can constitute a wide variety of materials, including ceramics, glass, polymers, and metals and can be synthesized by chemical process but wet processes for the preparation of microparticles have rarely been attemped. In this paper, a thrombotic route is shown to successfully generate biocompatible MP of a model anticancer drug (doxorubicin hydrochloride). Synthesis of MP s from platelets and drug loading in to these MP s was confirmed by flow cytometry and confocal microscopy. Human cervical cancer cell line (HeLa) was treated with these drug‐loaded MP s to investigate whether the loaded MP s have the capacity to deliver drug to the cancer cells. In addition, Magnetic force microscopy was used to detect the preparation of MP s loaded with magnetic NPs. The efficiency of the drug‐loaded MP s in inducing cytotoxicity in cancer cell line, shown to be significantly higher than the free drug itself. The drug‐loaded MP is shown to have a much higher cytotoxic propensity than the free drug applied at comparable doses. The thrombotic approach can also be applied to synthesize MP containing NPs which in turn can lead to generate a wide variety of new biocompatible materials.