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Crystallization and preliminary crystallographic analysis of a 2,3‐dihydroxybiphenyl dioxygenase from Pseudomonas sp. strain KKS102 having polychlorinated biphenyl (PCB)‐degrading activity
Author(s) -
Sugiyama Kazuyuki,
Narita Hiroki,
Yamamoto Takeshi,
Senda Toshiya,
Kimbara Kazuhide,
Inokuchi Norio,
Iwama Masanori,
Irie Masachika,
Fukuda Masao,
Yano Keiji,
Mitsui Yukio
Publication year - 1995
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340220309
Subject(s) - biphenyl , tetragonal crystal system , polychlorinated biphenyl , pseudomonas , strain (injury) , crystallization , dioxygenase , ammonium sulfate , chemistry , ammonium , crystallography , stereochemistry , crystal structure , organic chemistry , biology , bacteria , enzyme , anatomy , genetics
Abstract Crystals have been obtained for a 2,3‐dihydroxybiphenyl dioxygenase (conventionally called BphC) from a polychlorinated biphenyl (PCB)‐degrader, Pseudomonas sp. strain KKS1O2. The crystals were grown using both ammonium sulfate and MPD as the precipitating agents. The crystals belonged to a tetragonal space group ( I 422) and diffracted to 2.5 Å. © 1995 Wiley‐Liss, Inc.