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Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex
Author(s) -
Li Thomas,
Wolberger Cynthia,
Stark Martha,
Johnson Alexander D.
Publication year - 1995
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340210210
Subject(s) - crystallography , ternary operation , crystallization , diffraction , x ray crystallography , ternary complex , dna , base pair , x ray , base (topology) , chemistry , physics , optics , biochemistry , mathematical analysis , enzyme , mathematics , organic chemistry , computer science , programming language
Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P 6 1 or P 6 5 with a = b = 133 Å, c = 45.4 Å. Crystals that are flash‐frozen at −179°C diffract to 2.7 Å along the c ‐axis and to 2.4 Å in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. © 1995 Wiley‐Liss, Inc.