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Crystallization of the reaction center from Rhodobacter sphaeroides in a new tetragonal form
Author(s) -
Allen J. P.
Publication year - 1994
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340200309
Subject(s) - rhodobacter sphaeroides , photosynthetic reaction centre , tetragonal crystal system , crystallization , center (category theory) , chemistry , crystallography , stereochemistry , photochemistry , crystal structure , organic chemistry , photosynthesis , electron transfer , biochemistry
The reaction center from the nonsulfur purple bacterium Rhodobacter sphaeroides has been crystallized in a new form. The crystals grew in the presence of polyethylene glycol 4000, the detergent β‐octyl glucoside, and the amphiphiles heptane triol and benzamidine hydrochloride, using the sitting drop method. The space group of these crystals is tetragonal, P 4 1 (4 3 )2 1 2, and the cell constants are a = b = 141.5 Å and c = 276.7 Å with probably 2 proteins per asymmetric unit. A native data set has been set collected to a resolution of 2.8 Å consisting of 56,332 unique reflections (50,731 with F > 2σ) with an R sym of 9.5%. Analysis of the diffraction data is underway using molecular and isomorphous replacement. © 1994 Wiley‐Liss, Inc.