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Crystallographic studies and primary structure of the antitumor monoclonal CC49 Fab′
Author(s) -
Abergel Chantal,
Padlan Eduardo A.,
Kashmiri Syed V. S.,
Milenic Diane,
Calvo Benjamin,
Schlom Jeffrey
Publication year - 1993
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340170411
Subject(s) - monoclinic crystal system , immunoglobulin fab fragments , monoclonal antibody , immunoglobulin light chain , chemistry , molecule , crystallography , antibody , microbiology and biotechnology , crystal structure , stereochemistry , biology , immunology , complementarity determining region , organic chemistry
Abstract The Fab′ of CC49, a murine monoclonal antibody directed against the human tumor‐associated antigen TAG‐72 has been crystallized. The crystals are monoclinic, space group P 2 1 with cell parameters a = 115.6 Å, b = 116.4 Å, and c = 70.3 Å; β = 97.8°. The size of the unit cell is compatible with four Fab′ molecules in the asymmetric unit. The Fab molecules are related by two approximately perpendicular pseudo‐2‐fold axes. One pseudo‐2‐fold axis is parallel to the crystallographic 2‐fold axis and was found by inspection of the Harker section of the native Patterson map; the other was found by a self rotation function. The primary structures of the variable regions of the CC49 antibody light and heavy chains have been determined and are compared with those of the related antitumor antibody B72.3. © 1993 Wiley‐Liss, Inc.