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A monoclonal antibody fab fragment crystallized with and without a peptide epitope from HIV
Author(s) -
PfefferHennig Sabine,
Wessner Helga,
Hennig Michael,
Dauter Zbigniew,
Hausdorf Gert,
Höhne Wolfgang E.
Publication year - 1993
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340160411
Subject(s) - epitope , monoclonal antibody , orthorhombic crystal system , chemistry , peptide , microbiology and biotechnology , crystallization , antibody , stereochemistry , crystallography , biology , crystal structure , biochemistry , immunology , organic chemistry
The Fab fragment of CB 4‐1, a monoclonal murine antibody against HIV protein p24, has been produced. It forms a complex with a synthetic antigen, an epitope of p24 made up of 11 amino acids, with the binding constant k d = 3.6 × 10 −9 M. Crystals of hexagonal and orthorhombic space group has been obtained by cocrystallization of the Fab with the epitope and crystallization without the epitope, respectively. In either case, the crystals are suitable for X‐ray structural analysis. Crystals of the Fab fragment cocrystallized with the peptide have the space group P 6 3 22 with cell dimensions of a = b = 105 Å, c = 297 Å. Fab crystals without the epitope are in space group C 222 with cell dimensions a = 110.1 Å, b = 110.2 c Å = 150.1 Å. © 1993 Wiley‐Liss, Inc.