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Normal mode analysis of mouse epidermal growth factor: Characterization of the harmonic motion
Author(s) -
Ikura Teikichi,
Gō Nobuhiro
Publication year - 1993
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340160410
Subject(s) - motion (physics) , normal mode , terminal (telecommunication) , mode (computer interface) , bending , harmonic , physics , hinge , acoustics , classical mechanics , vibration , thermodynamics , engineering , computer science , operating system , telecommunications
Normal mode analysis of mouse epidermal growth factor (mEGF) has been carried out at room temperature. The value of the lowest frequency is 4.1 cm −1 . This mode corresponds to hinge‐bending motion between the N‐terminal and C‐terminal domains of mEGF. This hinge‐bending motion corresponds to the “mitten mode.” In this motion, the N‐terminal domain is almost rigid. However, the C‐terminal domain is found to consist of three rigid segments. Two segments, C33‐D46 and G51‐R53, are observed moving in the same direction, but L47‐W50 moves in the opposite direction. For this mode, the effective Young's modulus turned out to be 1.1 × 10 9 dyn· −2 . This value is a little larger than that of the mode with the lowest frequency 4.4 cm −1 of BPTI. The difference may be related to the fraction of residues involved in β‐sheets in the molecule. Similar analyses are carried out for other low frequency modes.